General Nitrotyrosine ELISA Kit

From Antibodies.com, this competitive Enzyme-Linked Immunosorbent Assay (cELISA) is designed for the in vitro quantitative determination of Nitrotyrosine in serum, plasma, tissue homogenates or other biological fluids. The kit has universal reactivity and is built around a pre-coated microplate in a 12 x 8 well strip format. Detection is colourmetric, read at 450 nm in a microplate reader, with a total assay time of 2h 30m.

Quantitative performance is defined by a sensitivity of <3.5 ng/ml and an assay range of 7.81–500 ng/ml. Reported average recoveries are 91% for serum (range 80–102%, n=5), 90% for EDTA plasma (range 81–99%, n=5) and 84% for heparin plasma (range 80–89%, n=5), with dilution linearity reported at 1:2, 1:4, 1:8 and 1:16 across serum, EDTA plasma and heparin plasma samples.

The competitive immunoassay principle uses a Biotinylated Anti-Nitrotyrosine Antibody and an HRP-Avidin conjugate; TMB substrate yields a yellow product whose intensity is inversely proportional to Nitrotyrosine concentration. Supplied components include the pre-coated plate, lyophilised standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers, with individual storage temperatures between +4°C and -20°C as instructed.