General Gamma-Aminobutyric Acid ELISA Kit

From Antibodies.com, this competitive Enzyme-Linked Immunosorbent Assay (cELISA) is designed for the in vitro quantitative determination of gamma Aminobutyric Acid in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The kit uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and relies on competition between antigen in the sample or standard and a fixed amount of immobilised gamma Aminobutyric Acid for binding sites on a Biotinylated Anti-gamma Aminobutyric Acid Antibody, with detection via HRP-Avidin and TMB substrate read at 450 nm on a colorimetric microplate reader.

Stated performance includes a product range of 31.2–2000 pg/ml and a sensitivity of <10.9 pg/ml, with a total assay time of 2 h 30 min. Kit components are stored at +4°C or -20°C as instructed. Reported spike-and-recovery averages are 91% for serum (n=5, range 80%–102%), 90% for EDTA plasma (n=5, range 81%–100%), and 84% for heparin plasma (n=5, range 80%–89%), with linearity ranges documented across sample dilutions of 1:2, 1:4, 1:8, and 1:16.