General Free Triiodothyronine ELISA Kit

This competitive ELISA from Antibodies.com quantifies free triiodothyronine (FT3), the unbound biologically active fraction of the T3 thyroid hormone. The kit uses a microplate-based competitive immunoassay format, supplied with enzyme-conjugated T3-HRP tracer, lyophilized calibrators, wash concentrate, and substrate solution.

Sample FT3 competes with the labeled tracer for binding to immobilized antibodies, producing an inverse absorbance signal. Each well requires 50 µl of test sample. Reconstituted standards should be refrigerated; the lyophilized antibody-coated plate and tracer vials are supplied ready to use and stored at room temperature until first opening.

Applications include thyroid function research, studies of hyperthyroidism and hypothyroidism, and endocrine assay development. Each single-use break-apart well strip, reagent vial, and protocol insert is intended for research use only, supporting quantitative FT3 measurement in clinical, veterinary, and basic science laboratories.