General Androstenedione ELISA Kit

From Antibodies.com, this competitive Enzyme-Linked Immunosorbent Assay (cELISA) is designed for the in vitro quantitative determination of Androstenedione with general reactivity. It is suitable for serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids, and is read colourmetrically on a pre-coated 96-well microplate configured as 12 x 8 well strips, with absorbance measured at 450 nm.

The kit covers a product range of 0.312–20 ng/ml with a sensitivity of 0.079 ng/ml, and the complete assay runs in 2 hours 30 minutes. Standards or samples compete with plate-bound Androstenedione for binding sites on a biotinylated anti-Androstenedione antibody; after washing, an HRP-avidin conjugate and TMB substrate produce a yellow colour whose intensity is inversely proportional to the analyte concentration, allowing quantification by reference to a standard curve. Validated recovery averaged 91% in serum (range 80%–102%, n=5), 90% in EDTA plasma (range 81%–100%, n=5) and 84% in heparin plasma (range 80%–89%, n=5), while dilution linearity at 1:2, 1:4, 1:8 and 1:16 spanned 74%–108% across all three sample types. Kit components should be stored at +4 °C or -20 °C as instructed and the kit must not be used past its expiration date.