General Allopregnanolone ELISA Kit

This competitive enzyme-linked immunosorbent assay (cELISA) from Antibodies.com is designed for the in vitro quantitative determination of Allopregnanolone in serum, plasma, or other biological fluids. The kit employs a pre-coated 96-well microplate (12 x 8 well strips) and a colorimetric detection principle, with absorbance read at 450 nm in a microplate reader.

During the assay, Allopregnanolone in samples or standards competes with immobilized antigen for binding sites on a biotinylated anti-Allopregnanolone antibody. Following incubation and washing, an HRP-Avidin conjugate is added. TMB substrate solution is then used to visualize the enzymatic reaction, producing a blue product that turns yellow upon addition of acidic stop solution, with colour density inversely proportional to Allopregnanolone concentration.

The assay has a measuring range of 1.56-100 ng/ml, a sensitivity of 0.49 ng/ml, and a total assay time of 2 hours 30 minutes. Reported recovery ranges are 80%-102% for serum, 81%-99% for EDTA plasma, and 80%-89% for heparin plasma. Kit components include the pre-coated microplate, lyophilized standard, detection solutions, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, stored at +4°C or -20°C as instructed.