General Adenosine Triphosphate ELISA Kit

The General Adenosine Triphosphate ELISA Kit from Antibodies.com offers a competitive enzyme-linked immunosorbent assay format intended to quantify ATP, the universal cellular energy currency in living cells. This 48T configuration supports research on mitochondrial respiration, glycolytic output, purinergic signaling events, and metabolic stress responses in cell lysates or tissue homogenates. Each kit includes a pre-coated microwell plate, calibrated ATP standards, anti-ATP competitive antibody, detection reagents, wash buffer, and substrate sufficient to run 48 determinations.

The competitive architecture lets sample ATP compete with plate-bound ATP analogue for antibody binding sites, enabling measurement of this small nucleotide without requiring paired capture and detection antibodies. A typical reaction uses 50 uL of prepared sample or standard per well, with absorbance read on a standard microplate reader. The kit supports intracellular ATP quantitation from cultured mammalian cells exposed to metabolic inhibitors, extracellular ATP released from activated immune cells, and ATP measurement in microbial cultures. Investigators studying drug-induced cytotoxicity, ischemic injury, or bioenergetic interventions will find quantitative ATP measurement a useful complement to luciferase-based luminescence assays, providing an antibody-based alternative that does not require specialized luminescent substrates. For research use only.