General 5-Hydroxytryptamine ELISA Kit

A competitive Enzyme-Linked Immunosorbent Assay (cELISA) from Antibodies.com designed for the in vitro quantitative determination of 5-Hydroxytryptamine in serum, plasma and other biological fluids. The assay is run on a pre-coated 96-well microplate arranged as 12 x 8 well strips and is read on a colorimetric microplate reader at 450 nm.

Key performance specifications include a sensitivity of 4.16 ng/ml and an assay range of 15.6-1000 ng/ml. The total assay time is approximately 2h 30m. The detection antibody is a rabbit IgG, and the assay uses a competitive inhibition enzyme immunoassay technique in which an HRP-Avidin conjugate produces a colorimetric signal inversely proportional to the analyte concentration in the sample.

Kit components should be stored at -20°C (with some items kept at +4°C as instructed), and the product is intended for research use only. Reactivity is classified as universal/general.