Equine Vascular Endothelial Growth Factor A ELISA Kit

Manufactured by Antibodies.com, this Sandwich ELISA kit is designed for the in vitro quantitative determination of Vascular Endothelial Growth Factor A (VEGFA) in equine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The assay uses a pre-coated 96-well microplate and employs the sandwich enzyme immunoassay technique, with a biotinylated anti-VEGFA antibody and an HRP-avidin conjugate. Detection is visualised via TMB substrate read at 450 nm on a colorimetric microplate reader.

Working range spans 31.2-2000 pg/ml with a reported sensitivity of <12.2 pg/ml, and the complete assay time is 4 hours 30 minutes. Reported recovery averages include serum at 91% (range 80-102%, n=5), EDTA plasma at 90% (range 81-100%, n=5), and heparin plasma at 84% (range 80-89%, n=5). Dilution linearity across 1:2 to 1:16 dilutions for serum ranged 74-107%, EDTA plasma 79-108%, and heparin plasma 82-105%.

Kit components supplied by the manufacturer include the pre-coated 96-well microplate, lyophilised standard, Detection Solutions A and B, Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers. Storage instructions direct users to store components at +4°C or -20°C as labelled and to refrain from use past the kit expiration date.