Equine Alpha-Fetoprotein ELISA Kit

The Antibodies.com Equine Alpha-Fetoprotein ELISA Kit is a sandwich-format 96T microplate assay for quantitative measurement of AFP in equine samples. Researchers investigating equine reproductive biology, fetal development, and hepatic function use this assay to quantify alpha-fetoprotein as a serum biomarker in mares and neonatal foals.

The kit includes a pre-coated 96-well plate, biotinylated detection antibody, streptavidin-HRP conjugate, lyophilized AFP standards, wash buffer, and TMB substrate. A standard curve spans 1.56 to 100 ng/ml, with analytical sensitivity near 0.78 ng/ml. Each well requires a 50 ul sample, and the full protocol can be completed within roughly 3 hours at room temperature. The capture and detection antibodies are specific to equine AFP, with no significant cross-reactivity reported against human, mouse, or bovine alpha-fetoprotein, supporting its use as a species-specific pregnancy and liver-function marker in horses.