EPHA2 + 3 + 4 (phospho Tyr588 + Tyr596) Cell Based ELISA Kit

Manufactured by Antibodies.com, this Cell-Based ELISA Kit is an Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of EphA2, EphA3, and EphA4 levels in adherent and suspension cells. The assay shows reactivity to Human (Tyr588 + Tyr596), Mouse (Tyr589 + Tyr596), and Rat (- + Tyr597) samples, with no reported cross-reactivity.

The kit uses a colorimetric detection method on a Tissue Culture Treated Microplate platform, with a total assay time of 4 hours 30 minutes. It targets the phospho Tyr588 + Tyr596 modification. Capture is performed using anti-EPHA2 + 3 + 4 antibodies, which are then detected by HRP-conjugated secondary antibodies, producing a colorimetric signal upon substrate addition.

Several normalization strategies are supplied with the kit: an Anti-GAPDH antibody serves as an internal positive control, Crystal Violet whole-cell staining is used to determine cell density, and a non-phosphorylated Anti-EPHA2 + 3 + 4 antibody can be used to normalize the phosphorylated signal. The product range is > 5000 Cells, and the kit should be stored at +4°C, where it remains stable for 3-6 months.