Commassie Brilliant Blue R-250, Cas # 6104-59-2

This triphenylmethane protein dye is supplied by MOLEQULE-ON, New Zealand (CAS 6104-59-2) and is primarily used for the visualization of protein bands after electrophoresis. It forms intensely coloured complexes with proteins, producing an intense blue colour on binding, and is widely employed for routine SDS-PAGE gel staining because of its good sensitivity and reversible binding characteristics.

The product is a dark purplish/blue powder, soluble to give dark blue/black solutions in aqueous and acidic organic media. Its molecular weight is approximately 826 g·mol⁻¹, and under typical protocols it is reported to achieve detection of protein bands in gels down to around 0.5 μg/cm². It is intended for laboratory research use only and is not for diagnostic or therapeutic applications.

For handling, the dye should be stored dry, protected from strong light and moisture at ambient temperature. Staining and destaining solutions should be prepared freshly, and any solvent-based waste disposed of in accordance with local regulations. Users are advised to follow the supplied Safety Data Sheet and standard laboratory safety practices.