Chicken Netrin 1 ELISA Kit

Manufactured by Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is intended for the in vitro quantitative determination of chicken Netrin 1 in serum, plasma or other biological fluids. The assay uses a pre-coated 96-well microplate and a biotinylated anti-Netrin 1 detection antibody, with an HRP-avidin conjugate and TMB substrate; absorbance is read at 450 nm on a colorimetric microplate reader.

According to the manufacturer, the working range is 15.6-1000 pg/ml and the sensitivity is <5.4 pg/ml, with a total assay time of 4h 30m. Reported ranges across five replicates are 88-101% in serum, 91-103% in EDTA plasma, and 92-98% in heparin plasma. Kit components should be stored at +4°C or -20°C as instructed and not used past the expiration date.