Chicken Nerve Growth Factor ELISA Kit

From Antibodies.com, this Chicken Nerve Growth Factor ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of chicken NGF in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

The kit uses a pre-coated 96-well microplate (12 x 8 well strips) together with a biotinylated anti-NGF antibody and HRP-avidin detection with TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader. The stated product range is 31.2-2000 pg/ml and sensitivity is 10.6 pg/ml. Total assay time is 4h 30m, and kit components are stored at +4°C or -20°C as instructed.

Spike-and-recovery results (n=5) gave averages of 91% in serum (range 80-102%), 90% in EDTA plasma (range 81-100%), and 84% in heparin plasma (range 80-89%). Dilution data (n=5) across 1:2, 1:4, 1:8, and 1:16 dilutions were 87-91%, 87-107%, 74-101%, and 92-97% in serum; 90-105%, 84-101%, 90-101%, and 79-108% in EDTA plasma; and 84-95%, 92-105%, 82-105%, and 89-91% in heparin plasma.