Chicken Mannose Binding Lectin ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay is intended for the in vitro quantitative determination of chicken Mannan Binding Lectin/MBL in serum, plasma or other biological fluids. The kit uses a pre-coated 96-well microplate in a 12 x 8 well strip format, with colorimetric detection read at 450 nm on a standard microplate reader.

The assay reports a sensitivity of <0.57 ng/ml and a working range of 1.56-100 ng/ml, with a total run time of 4 hours 30 minutes. Capture is performed by an antibody specific for Mannan Binding Lectin/MBL, followed by a biotinylated detection antibody, HRP-Avidin and TMB substrate, with an acidic stop solution producing a yellow colour proportional to the amount of target captured in each well.

Sample recovery was evaluated in serum, EDTA plasma and heparin plasma matrices, giving average recoveries of 91% (n=5, range 80%-102%), 90% (n=5, range 81%-100%) and 84% (n=5, range 80%-89%) respectively. Provided components include a pre-coated microplate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers. Kit components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.