Chicken Interleukin 18 ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay is designed for the in vitro quantitative determination of chicken Interleukin 18 (IL-18) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Listed under the Assays and Kits category, the kit reacts with chicken samples and is built around a pre-coated 96-well microplate in a 12 x 8 well strip format. A biotinylated anti-IL-18 detection antibody and HRP-avidin conjugate are used, with a TMB substrate step read at 450 nm on a colourmetric microplate reader.

The assay reports a standard range of 15.6–1000 pg/ml, a sensitivity of <7.3 pg/ml, and a total run time of 4 h 30 min. Validation work (n=5) records average recovery of 91% from serum, 96% from EDTA plasma, and 95% from heparin plasma. Dilution linearity tested at 1:2, 1:4, 1:8 and 1:16 spans 73% to 110% across the same sample types. Components are stored at +4 °C or −20 °C as instructed, and the kit should not be used past its expiration date.