Chicken Fibroblast Growth Factor 4 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com enables the in vitro quantitative determination of chicken Fibroblast Growth Factor 4 (FGF4). The kit is intended for serum, plasma and other biological fluids and is run on a pre-coated microplate with a total assay time of 4 hours 30 minutes.

Samples are incubated with a biotinylated anti-FGF4 antibody, followed by an HRP-avidin conjugate and TMB substrate. The resulting colour change is read spectrophotometrically at 450 nm in a colorimetric microplate reader. Sensitivity is reported at 6.9 pg/ml and the assay range at 15.6–1000 pg/ml, with one external listing giving the range as 15.625–1000 pg/ml. The capture antibody is described as a mouse IgG isotype reagent, and the product targets Gallus reactivity.

Kit components should be stored at +4°C or -20°C according to the manufacturer's instructions, and the kit should not be used past its expiration date.