Chicken Complement Component 3 ELISA Kit

Antibodies.com offers a sandwich ELISA kit for the in vitro quantitative determination of chicken Complement C3 in serum, plasma, or other biological fluids. The assay uses an antibody specific for Complement C3 pre-coated onto a microplate, with captured analyte detected using a biotinylated anti-Complement C3 antibody and HRP-avidin conjugate, then visualised with TMB substrate that turns yellow upon addition of the acidic stop solution.

The kit provides a quantitative measurement range of 31.2-2000 ng/ml with a sensitivity of <11.2 ng/ml. Detection is colorimetric, with absorbance read at 450 nm in a microplate reader and concentration calculated from a standard curve. The assay is performed on a pre-coated microplate arranged as 12 x 8 well strips, with a total assay time of approximately 4 hours 30 minutes.

Kit components include a pre-coated 96-well microplate, lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers. Components should be stored at +4°C or -20°C as indicated on their labels, and the kit must not be used past its expiration date. Reported recovery values are 80-102% in serum (n=5, average 91%), 81-99% in EDTA plasma (n=5, average 90%), and 80-89% in heparin plasma (n=5, average 84%).