Chicken Ciliary Neurotrophic Factor ELISA Kit

This sandwich ELISA kit from Antibodies.com enables the in vitro quantitative determination of chicken Ciliary Neurotrophic Factor (CNTF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids [S1]. The assay is run on a 96-well microplate pre-coated with an antibody specific for CNTF. Captured analyte is detected using a Biotinylated Anti-CNTF Antibody and an HRP-Avidin conjugate, with a TMB substrate step producing a yellow product whose intensity is read at 450 nm on a colorimetric microplate reader [S1].

According to the manufacturer, the kit has a product range of 62.5–4000 pg/ml and a sensitivity of 29.4 pg/ml, with a total assay time of 4 hours 30 minutes [S1]. Kit components should be stored at +4°C or -20°C as instructed and the kit must not be used past its expiration date [S1]. Recovery data for serum, EDTA plasma and heparin plasma, along with dilution linearity across multiple dilutions, are reported in the product documentation [S1].