Canine Transforming Growth Factor Beta 1 ELISA Kit

Antibodies.com supplies a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) for the in vitro quantitative determination of canine TGF beta 1 in serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids. The format uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, read at 450 nm on a colorimetric microplate reader, with a stated product range of 15.6-1000 pg/ml and a sensitivity of <5.6 pg/ml. Total assay time is 4h 30m.

Recovery has been validated in serum (range 80%-102%, average 91%, n=5), EDTA plasma (range 81%-100%, average 90%, n=5) and heparin plasma (range 80%-89%, average 84%, n=5). Dilution linearity was demonstrated at 1:2, 1:4, 1:8 and 1:16 across serum, EDTA plasma and heparin plasma. Supplied components include the pre-coated 96-well microplate, lyophilized standard (2 vials), Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers; components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.