Canine Tissue Inhibitors Of Metalloproteinase 2 ELISA Kit

Manufactured by Antibodies.com, this Canine TIMP2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of canine Tissue Inhibitors Of Metalloproteinase 2 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

The assay uses the sandwich enzyme immunoassay technique with a pre-coated 96-well microplate (12 x 8 well strips). Standards and samples are added to the wells, captured TIMP2 is detected with a Biotinylated Anti-TIMP2 Antibody followed by HRP-Avidin conjugate, and the reaction is visualised using TMB substrate solution with absorbance read at 450 nm in a Colorimetric Microplate Reader. The stated product range is 6.25-400 ng/ml with a sensitivity of 1.5 ng/ml, and the total assay time is 4h 30m. Manufacturer-reported spiking recovery values (n=5) are: Serum 80% - 102% (average 91%), EDTA Plasma 81% - 100% (average 90%), and Heparin Plasma 80% - 89% (average 84%).

Kit components include the pre-coated microplate, lyophilised standard, Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers, with storage at +4°C or -20°C as instructed.