Canine Regulated On Activation In Normal T-Cell Expressed And Secreted ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is intended for the in vitro quantitative determination of canine RANTES in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips), a biotinylated anti-RANTES detection antibody, an HRP-Avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

Manufacturer data states a standard range of 31.2-2000 pg/ml and a sensitivity of 7.8 pg/ml, with a total assay time of 4h 30m. Reported spike-and-recovery averages are 91% in serum (range 80%-102%, n=5), 90% in EDTA plasma (range 81%-100%, n=5), and 84% in heparin plasma (range 80%-89%, n=5). Dilution linearity at 1:2, 1:4, 1:8 and 1:16 has been characterised for serum, EDTA plasma and heparin plasma. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.