Canine Osteopontin ELISA Kit

From Antibodies.com, this sandwich ELISA is designed for the in vitro quantitative determination of canine Osteopontin in serum, plasma, tissue homogenates or other biological fluids. The assay employs pre-coated microplate wells (12 x 8 well strips) with an antibody specific for Osteopontin. Standards and samples are added to the wells and captured Osteopontin is detected using a Biotinylated Anti-Osteopontin Antibody followed by HRP-Avidin conjugate. TMB substrate is used to visualise the enzymatic reaction, producing a blue colour that changes to yellow upon addition of stop solution. The yellow colour density, proportional to the Osteopontin concentration, is read as optical density at 450 nm using a colorimetric microplate reader.

The assay has a reported sensitivity of <0.61 ng/ml and a working range of 1.56-100 ng/ml, with a total assay time of 4h 30m. Kit components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.

Reported spike-and-recovery averages in the validation data are 91% in serum, 90% in EDTA plasma, and 84% in heparin plasma, with dilution linearity reported across 1:2 to 1:16 dilutions for each sample type.