Canine Matrix Metalloproteinase 3 ELISA Kit

Manufactured by Antibodies.com and listed under its Assays and Kits category, this sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit is designed for the in vitro quantitative determination of canine matrix metalloproteinase 3 (MMP3) in serum, plasma, tissue homogenates or other biological fluids. The plate is pre-coated with an antibody specific for MMP3; bound analyte is detected with a biotinylated anti-MMP3 antibody and an HRP-avidin conjugate, with signal developed using a TMB substrate and read as absorbance at 450 nm on a colorimetric microplate reader.

The stated assay range is 0.156–10 ng/ml with a sensitivity of <0.063 ng/ml, and the total assay time is 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as instructed, and the product should not be used past its expiration date. The application is reported as canine-reactive sandwich ELISA only.

Manufacturer-reported spike-and-recovery values, tested at multiple sample dilutions (1:2, 1:4, 1:8 and 1:16), were: serum 80%–102% (average 91%), EDTA plasma 81%–100% (average 90%), and heparin plasma 80%–89% (average 84%).