Canine Interleukin 2 ELISA Kit

From Antibodies.com, this Canine Interleukin 2 ELISA Kit is a sandwich enzyme-linked immunosorbent assay designed for the quantitative measurement of canine IL-2 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids [S1]. The assay uses a pre-coated 96-well microplate and is read on a colorimetric microplate reader at 450 nm, with absorbance values used to calculate IL-2 concentration against a standard curve [S1]. The stated product range is 15.6–1000 pg/ml and the reported sensitivity is <6.2 pg/ml [S1]. The total assay time is 4 hours 30 minutes [S1].

Reported spike-and-recovery averages across five samples per matrix are 91% for serum, 90% for EDTA plasma and 84% for heparin plasma [S1]. Kit components include a pre-coated 96 well microplate in a 12 x 8 well strip format, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers [S1]. Components are stored at +4°C or -20°C as specified in the protocol, and the kit should not be used past its expiration date [S1].