Canine Hepatocyte Growth Factor ELISA Kit

Antibodies.com supplies a sandwich ELISA for the in vitro quantitative determination of canine HGF in serum, plasma or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with detection via a biotinylated anti-HGF antibody and an HRP-avidin conjugate visualised using TMB substrate; the colourmetric reaction is read at 450 nm.

According to the manufacturer, the kit reports a sensitivity of <5.6 pg/ml and an assay range of 15.6-1000 pg/ml. Total assay time is 4h 30m. Kit components — pre-coated microplate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers — are stored at +4°C or -20°C as instructed.

Manufacturer-reported spike-and-recovery averages in canine samples are 91% for serum (range 80%-102%), 90% for EDTA plasma (range 81%-99%) and 84% for heparin plasma (range 80%-89%).