Canine Glucose Transporter 4 ELISA Kit

Manufactured by Antibodies.com, this is a sandwich ELISA for the in vitro quantitative determination of canine Glucose Transporter GLUT4 in tissue homogenates, cell lysates, or other biological fluids. The assay employs a pre-coated microplate, a biotinylated anti-GLUT4 detection antibody, and an HRP-avidin conjugate with TMB substrate, producing a colourmetric end-product read at 450 nm in a microplate reader.

According to the manufacturer's documentation, the kit has a sensitivity of 0.121 ng/ml and a working range of 0.312–20 ng/ml, with a total assay time of 4 h 30 min. Kit components are stored at +4 °C or −20 °C as indicated.

Validation data are reported for serum, EDTA plasma, and heparin plasma samples. Average recovery values across the tested dilution series (1:2 to 1:16) are 91% for serum, 90% for EDTA plasma, and 84% for heparin plasma, with individual dilution ranges provided in the manufacturer's documentation.