Canine Connexin 43 ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA is designed for the in vitro quantitative determination of canine Connexin 43/GJA1 in tissue homogenates, cell lysates, or other biological fluids. The format uses a 96-well pre-coated microplate arranged as 12 x 8 well strips, with an antibody specific for Connexin 43/GJA1 immobilized on the wells. Detection uses a biotinylated anti-Connexin 43/GJA1 antibody, an HRP-avidin conjugate, and a TMB substrate, with absorbance read at 450 nm in a colorimetric microplate reader.

According to the manufacturer's data, the kit has a sensitivity of <0.074 ng/ml, an assay range of 0.312-20 ng/ml, and a total assay time of 4h 30m. Components include a pre-coated microplate, lyophilized standard, Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and adhesive plate sealers. Storage is at +4°C or -20°C as appropriate.

Manufacturer-reported spike-and-recovery values (n=5) are 80%-102% averaging 91% in serum, 81%-100% averaging 90% in EDTA plasma, and 80%-89% averaging 84% in heparin plasma. Dilution linearity at 1:2 to 1:16 is provided for all three sample types.