Bovine Xanthine Dehydrogenase ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (sELISA) kit from Antibodies.com is intended for the in vitro quantitative determination of bovine Xanthine Oxidase in serum, plasma, tissue homogenates or other biological fluids. The catalogue entry lists the target as Xanthine Dehydrogenase and indicates reactivity against bovine samples. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and employs the sandwich immunoassay technique with an antibody specific to the target, a biotinylated detection antibody and an HRP-Avidin conjugate, with signal developed using TMB substrate and read at 450 nm on a colorimetric microplate reader.

According to the manufacturer's protocol, the assay range is 15.6–1000 pg/ml with a sensitivity of <6.3 pg/ml, and total assay time is 4 h 30 min. Recovery ranges of 80–102% (serum, n=5), 81–99% (EDTA plasma, n=5) and 80–89% (heparin plasma, n=5) are reported. Kit components are to be stored at +4°C or -20°C as instructed and not used past the stated expiration date.