Bovine Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is designed for the in vitro quantitative determination of bovine TIE1 (Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. It is reactive with bovine samples and is read on a colorimetric microplate reader at 450 nm.

The assay uses a pre-coated 96-well microplate in a 12 x 8 well strip format and follows a biotinylated anti-TIE1 antibody / HRP-avidin / TMB substrate detection scheme, with an assay time of 4h 30m. The product range is 31.2-2000 ng/ml, with a stated sensitivity of <11.32 ng/ml. Storage instructions call for kit components to be kept at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.

Reported recovery values are 86% - 100% (average 93%) for serum (n=5), 87% - 101% (average 94%) for EDTA plasma (n=5), and 88% - 100% (average 94%) for heparin plasma (n=5), with corresponding dilution linearity ranges across 1:2, 1:4, 1:8 and 1:16 sample dilutions.