Bovine Tumor Necrosis Factor Beta ELISA Kit

A sandwich ELISA from Antibodies.com intended for the in vitro quantitative determination of bovine LTA — identified in the catalogue listing under the target name Tumor Necrosis Factor Beta — in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The assay employs the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate arranged as 12 x 8 well strips, with an antibody specific for the target immobilised on the plate. Standards and samples are added, followed by a Biotinylated Anti-LTA Antibody and then an HRP-Avidin conjugate. TMB substrate is used to visualise the enzymatic reaction, producing a blue-coloured product that changes to yellow upon addition of acidic stop solution; the yellow intensity is proportional to the amount of LTA captured in each well, read as O.D. absorbance at 450 nm in a microplate reader and referred to a standard curve.

The product range is 0.156-10 ng/ml with a sensitivity of <0.041 ng/ml, and the total assay time is 4h 30m. Reported average recovery values are 80%-102% for serum (n=5, mean 91%), 81%-100% for EDTA plasma (n=5, mean 90%), and 80%-89% for heparin plasma (n=5, mean 84%), with linearity data provided across 1:2, 1:4, 1:8 and 1:16 sample dilutions for each matrix.

Kit components include a pre-coated 96-well microplate, lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers. Components should be stored at +4°C or -20°C as instructed and must not be used past the expiration date.