Bovine Tumor Necrosis Factor Alpha ELISA Kit

From Antibodies.com, this Sandwich ELISA is designed for the in vitro quantitative determination of bovine Tumor Necrosis Factor Alpha (TNF alpha) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, or other biological fluids. The assay uses the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate (12 x 8 well strips), with detection performed on a colorimetric microplate reader by measuring absorbance at 450 nm.

The stated product range is 78.1-5000 pg/ml, with a sensitivity of <32 pg/ml. Total assay time is 4h 30m. Kit components include a pre-coated 96-well microplate (12 x 8 well strips), lyophilized standard, Detection Solution A (120 μl), Detection Solution B (120 µl), 30X Wash Buffer (20 ml), Sample Dilution Buffer (45 ml), TMB Substrate (9 ml), Stop Solution (6 ml), and adhesive plate sealers. Components should be stored at +4°C or -20°C as instructed.

Reported average recovery values (n=5) are 91% for serum (range 80%-102%), 90% for EDTA plasma (range 81%-100%), and 84% for heparin plasma (range 80%-89%). Reported dilution linearity across 1:2 to 1:16 dilutions spans 87-107% for serum, 84-108% for EDTA plasma, and 82-105% for heparin plasma.