Bovine Toll Like Receptor 4 ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables the in vitro quantitative determination of bovine TLR4 in serum, plasma, tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and a biotinylated anti-TLR4 antibody with HRP-avidin detection, visualised using TMB substrate and read at 450 nm on a colorimetric microplate reader.

With an assay range of 0.625-40 ng/ml and a sensitivity of 0.237 ng/ml, the quantitative sandwich format takes 4h 30m to complete. Reported recovery ranges are 80-102% for serum, 81-100% for EDTA plasma, and 80-89% for heparin plasma. Kit components are stored at +4°C or -20°C as instructed and must not be used past the expiration date.