Bovine Ribonuclease A ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of bovine Ribonuclease A in serum, plasma, tissue homogenates and other biological fluids. The 96-well microplate is supplied pre-coated and arranged as 12 x 8 well strips. The workflow uses a biotinylated anti-Ribonuclease A detection antibody followed by HRP-avidin and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader. The product sits in the Assays and Kits category with reactivity against bovine samples and a target of Ribonuclease A.

Reported performance figures include a sensitivity of <0.66 ng/ml, an assay range of 1.56–100 ng/ml and a total assay time of 4 hours 30 minutes. Recovery has been characterised in serum (average 91%, range 80–102%, n=5), EDTA plasma (average 90%, range 81–100%, n=5) and heparin plasma (average 84%, range 80–89%, n=5), with dilution linearity reported across 1:2 to 1:16 sample dilutions for each matrix. Components are stored at +4°C or –20°C as instructed and the kit should not be used past its expiration date.