Bovine Regulated On Activation In Normal T-Cell Expressed And Secreted ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay is designed for the in vitro quantitative determination of bovine RANTES (Regulated on Activation, Normal T-Cell Expressed and Secreted) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips. Standards and samples are captured by an immobilised anti-RANTES antibody, detected with a biotinylated anti-RANTES antibody and an HRP-avidin conjugate, and visualised using a TMB substrate; the catalytic reaction yields a blue product that turns yellow after addition of the acidic stop solution, with absorbance read at 450 nm on a colorimetric microplate reader.

The stated product range is 78.1–5000 pg/ml with a sensitivity of <18.8 pg/ml, and the total assay time is approximately 4 hours 30 minutes. Reported spike-and-recovery performance is: serum, range 80%–102%, average 91% (n=5); EDTA plasma, range 81%–100%, average 90% (n=5); and heparin plasma, range 80%–89%, average 84% (n=5). The manufacturer states the kit is suitable for serum, plasma, cell lysates, tissue homogenates and cell culture supernates. Kit components should be stored at +4 °C or −20 °C as instructed, and the kit should not be used past its expiration date.