Bovine Lactoperoxidase ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of bovine lactoperoxidase (LPO) in milk, tears and other biological fluids. The assay has a detection range of 0.156–10 ng/ml with a sensitivity of 0.058 ng/ml and an assay time of 4h 30m, run on a pre-coated microplate with absorbance read at 450nm. A biotinylated anti-LPO detection antibody is used with an HRP-avidin conjugate and TMB substrate, producing a coloured product whose intensity is proportional to the LPO captured in each well.

Acceptable sample types include milk, tears and other biological fluids. Reported recovery across serum (n=5), EDTA plasma (n=5) and heparin plasma (n=5) averages 91%, 90% and 84% respectively, with corresponding ranges of 80%–102%, 81%–99% and 80%–89%. Dilution linearity has been demonstrated at 1:2, 1:4, 1:8 and 1:16 in serum, EDTA plasma and heparin plasma. According to the third-party listing, the antibody host is rabbit and the isotype is IgG. Kit components should be stored at +4°C or -20°C as instructed, and the kit must not be used past its expiration date.