Bovine Interferon Inducible T-Cell Alpha Chemoattractant ELISA Kit

This Antibodies.com sandwich ELISA kit is designed for the in vitro quantitative determination of bovine CXCL11 (Interferon Inducible T-Cell Alpha Chemoattractant) in serum, plasma, or other biological fluids. The assay uses a pre-coated microplate (12 x 8 well strips) with colourimetric detection read at 450 nm, and the complete protocol runs in 4 hours 30 minutes.

Performance is defined by a measuring range of 46.8–3000 pg/ml and a sensitivity of <15.46 pg/ml. In validation testing (n=5), recovery averaged 91% in serum (80–102%), 90% in EDTA plasma (81–100%) and 84% in heparin plasma (80–89%), with consistent values reported across dilutions from 1:2 to 1:16. The workflow pairs a biotinylated anti-CXCL11 detection antibody with an HRP-avidin conjugate and TMB substrate, and kit components are stored at +4°C or −20°C as instructed.