Bovine Insulin Like Growth Factor Binding Protein 2 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com designed for the in vitro quantitative determination of bovine Insulin Like Growth Factor Binding Protein 2 (IGFBP2) in serum, plasma, tissue homogenates, or other biological fluids. The kit uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and a biotinylated detection antibody with HRP-avidin and TMB substrate. Results are read colorimetrically at 450 nm, with a sensitivity of 33 pg/ml and a detection range of 78.1-5000 pg/ml. Total assay time is 4 hours 30 minutes.

Manufacturer-reported recovery averaged 91% in serum (range 80%-102%), 90% in EDTA plasma (81%-100%), and 84% in heparin plasma (80%-89%), each across n=5 samples. Dilution linearity is reported across 1:2 to 1:16 dilutions for all three sample types. Supplied components include lyophilized standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers, with storage at +4°C or -20°C depending on the component.

Third-party information describes the capture antibody as a rabbit IgG, the kit as a liquid formulation, and lists the synonyms S14 and EMTB. The immunogen is described as a synthetic peptide of human RPS14. All products are sold for laboratory research use only and are not intended for human or animal therapeutic or diagnostic applications.