Alkaline Phosphatase Substrate Tabs, pk

A convenient chromogenic substrate for visualizing endogenous alkaline phosphatase (AP) activity in cultured cells, this pack of pre-weighed tablets from STEMCELL Technologies dissolves cleanly in buffer to produce a ready-to-use staining solution. Because AP expression is a classical hallmark of the undifferentiated state, the substrate is widely applied to human embryonic stem cells (hESCs), induced pluripotent stem cells (iPSCs), and reprogrammed colonies during routine pluripotency screening and quality control workflows.

Once dissolved, the working solution reacts with cellular AP to deposit a localized colored precipitate at sites of enzyme activity, allowing undifferentiated colonies to be scored quickly under a standard inverted microscope. The single use tablet format removes the variability of handling bulk powder and supports reproducible staining across multiple wells, dishes, and chamber slides in cell culture maintenance and differentiation protocols.

Typical use involves fixing cells, incubating with the prepared substrate at room temperature for 20 to 30 minutes, rinsing, and then evaluating color intensity. The pack should be stored dry and protected from light to preserve enzymatic reactivity through the stated shelf life, and the substrate integrates naturally into research use only workflows that characterize pluripotent cell lines and monitor reprogramming efficiency.