Absolute Master Mix (2X Ready Mix for conventional PCR)

A ready-to-use 2X PCR reaction mixture from MOLEQULE-ON, New Zealand, intended for conventional PCR. The reaction is set up by adding primers, DNA template and water to the mix. The formulation contains recombinant thermostable DNA polymerase, PCR buffer with Mg²⁺, dNTPs and loading dyes, and is supplied at 2X concentration so that approximately 50% of the final reaction volume can be used for primer and template solutions. Components are tested for the absence of DNase, RNase and exonuclease activities, and the recombinant Taq DNA polymerase is additionally tested for the absence of exonuclease and double- and single-stranded endonuclease activities.

Manufacturer-reported features include a high extension speed, completing a 2 kb target in 60 seconds (extension rate of 30–50 bases per second), high sensitivity for low-copy template amplification, amplification of long targets up to 5 kb from Lambda DNA, and stability for 21 days at 37 °C while maintaining the same performance. Loading dyes are included, allowing the PCR product to be loaded directly onto an agarose gel and visualised under UV or blue light. The product can be stored at 25 °C for up to one week, at 4 °C for up to six months and at −20 °C for up to one year. Suggested applications include routine conventional PCR, colony PCR, and amplification from plasmid or genomic templates.

For laboratory use only. Not intended for any animal or human therapeutic or diagnostic use. Because PCR can amplify trace amounts of DNA, all appropriate precautions should be taken to avoid cross-contamination, ideally by assembling amplification reactions in a DNA-free environment with filter tips and analysing PCR products in an area separate from the reaction preparation area.