ABL1 (phospho Thr735) Cell Based ELISA Kit

From Antibodies.com, this qualitative Cell-Based ELISA Kit enables indirect in vitro detection of c-Abl (ABL1) at the phospho Thr735 site in adherent and suspension cells. It is reactive with Human (Thr735), Mouse (Thr734) and Rat (Thr753) samples, with no cross-reactivity reported. The kit is part of a supplier portfolio of 7 c-Abl ELISA kits offering qualitative or quantitative detection across human, mouse and rat samples.

The assay uses a colorimetric indirect ELISA format in which the phospho-target is captured by anti-ABL1 (phospho Thr735) antibodies and detected using HRP-conjugated secondary antibodies. It is run on a Tissue Culture Treated Microplate over 4 hours 30 minutes, with a recommended cell input of > 5000 Cells.

Three normalisation strategies are described in the protocol: an included anti-GAPDH antibody to serve as an internal positive control; Crystal Violet whole-cell staining to correct for differences in plating and cell density; and an anti-ABL1 antibody for normalising the phosphorylated signal against the total target. The kit is stored at +4°C, where the supplier datasheet describes a shelf life of 3–6 months, while the third-party listing cites 6 months.