50bp DNA Ladder

From MOLEQULE-ON, this ready-to-use molecular weight marker is composed of PCR products and proprietary plasmids digested with restriction enzymes to yield 17 fragments ranging from 50 to 1,500 base pairs. Suitable for both horizontal and vertical gel electrophoresis, it features bands of increased intensity at 200 and 500 base pairs to serve as reference points. The approximate mass of DNA in each band is 0.56 µg per load, allowing estimation of DNA mass in comparably intense samples of similar size. Orange G is included as a tracking dye.

The DNA fragments are phenol-extracted and equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA. The recommended loading quantity is 5 µl of marker per well. Storage is at 4°C, or at –20°C for longer-term use. The product is supplied at a concentration of 112 µg/ml and is intended for laboratory use only.