Quantitative measurement of human thiosulfate sulfurtransferase (TST) is straightforward with this photometric microplate immunoassay from Assay Genie. The kit targets a mitochondrial sulfurtransferase enzyme that participates in cyanide detoxification and sulfur metabolism pathways, providing researchers with a reliable tool for biomarker investigations in toxicology, mitochondrial function studies, and xenobiotic metabolism research.

The procedure follows an indirect detection workflow: prepared samples and reference standards are added to antibody-coated wells, incubated, and washed. A biotinylated detection reagent binds the captured target, followed by a streptavidin-enzyme conjugate and a TMB substrate reaction. Signal intensity scales with TST concentration in each well, allowing interpolation of unknown sample values against the supplied standard curve.

Supplied components include the antibody-coated strip plate, lyophilized reference standard vials, biotinylated detection reagent, enzyme conjugate working solution, concentrated wash buffer, assay diluent, TMB substrate, stop reagent, and a sealed protocol insert with recommended dilution factors. The microplate layout accommodates flexible processing of partial runs alongside blanks, standards, and unknowns.

Common research contexts include toxicology studies examining sulfide and cyanide exposure, mitochondrial biomarker panels in metabolic and neurodegenerative disease research, and investigations into sulfur transferase activity in cell culture supernatant or tissue-derived specimens. The immunoassay format suits batched analysis of larger clinical or preclinical cohorts where consistent inter-assay performance supports longitudinal comparisons.

Storage recommendations involve keeping unopened components refrigerated until first use, with the reconstituted standard aliquoted and frozen to preserve activity across multiple runs. Repeated freeze-thaw cycles should be avoided to maintain standard curve integrity.

Consumables

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