Trypsin Digestion Solution, 0.25%

Trypsin Digestion Solution, 0.25% — MOLEQULE-ON (New Zealand). This product is an enhanced description of the original Trypsin Digestion Solution, 0.25%: sterile, ready-to-use liquid trypsin for cell dissociation and passaging. Size: 100 ml. Supplier: EMDA.

Manufacturer information & formulation: Recombinant, high-purity trypsin produced by MOLEQULE-ON (New Zealand); formulated at 0.25% (w/v) for routine detachment of adherent mammalian cells and provided without animal-derived raw materials to reduce adventitious-agent risk. (molequle-on.com)

• Key features: Ready-to-use liquid format; high specific activity for efficient detachment; reduced risk of animal-origin contaminants; suitable for primary cells and cell lines.
• Typical applications: Detachment/harvest of adherent mammalian cells, subculturing, preparation of single-cell suspensions for assays, and workflows in recombinant protein or vaccine production. (thermofisher.cn)
• Handling notes: Pre-warm reagent and balanced salt solutions as appropriate, rinse monolayers with Ca2+/Mg2+-free buffer before applying trypsin, monitor exposure time to avoid over-digestion, and neutralize with serum-containing medium or a validated neutralizing solution. Follow cell-line–specific recommendations. (sigmaaldrich.com)
• Storage & safety: Store and use according to manufacturer instructions; handle as an enzymatic reagent (use appropriate PPE, avoid inhalation/contact, and follow institutional biosafety procedures).

This enhanced description expands on the original entry by clarifying formulation, intended uses, and handling essentials while preserving the original product name and concentration. For specific protocol parameters and storage/expiry details, consult MOLEQULE-ON product documentation or contact the supplier EMDA. (molequle-on.com)