Trypan Blue, 100mL

Trypan Blue, 100 mL — Viability exclusion dye for manual and automated cell counting; dead cells take up the dye while live cells exclude it. Manufacturer: STEMCELL Technologies. Typical protocol: mix equal volumes of cell suspension and trypan blue, count immediately using a hemocytometer or automated counter. Handling: prepare fresh dilutions, avoid prolonged exposure to cells, store at room temperature away from direct light; follow institutional waste and disposal guidelines.