Murashige and Skoog Medium, Powder to make 1L media

Murashige and Skoog Medium, Powder to make 1L media

Murashige and Skoog Medium, Powder to make 1L media — powdered MS basal medium concentrate for preparation of 1 L plant tissue culture media. Manufacturer: MOLEQULE-ON (New Zealand). Supplier: EMDA. (Enhances the provided product description with specifications and usage notes.)

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• Key specifications: Powdered basal salts and vitamins formulated for plant tissue culture; typical reconstitution ~4.4 g powder per 1 L of medium (prepare with sterile water and supplement as required).
• Composition highlights: balanced macronutrients (high nitrate), micronutrients and vitamins (e.g., myo‑inositol, thiamine, nicotinic acid, pyridoxine) to support callus, cell suspension and organogenesis.

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Typical applications: callus induction and maintenance, cell suspension culture, shoot regeneration and micropropagation for research and commercial propagation of ornamental, vegetable and fruit species.

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Preparation & handling: dissolve the powder in distilled water, adjust pH (commonly pH 5.7) before sterilization, add sucrose, agar or plant growth regulators (auxins/cytokinins) as required; sterilize by autoclaving and add heat‑labile components after sterilization.

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Storage & safety notes: powdered media are hygroscopic — store sealed in a desiccator or refrigerated (0–5°C) and protect from moisture; use promptly after opening. Follow laboratory biosafety and waste disposal procedures when preparing and using culture media.

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