MQ Western One Step Blocker

MQ Western One Step Blocker

MQ Western One Step Blocker — MQ Western One Step Blocker is an enhanced, ready-to-use, protein‑free blocking solution that builds on the original product name and purpose. It combines membrane blocking with primary and secondary antibody incubation in a single step to reduce hands‑on time while lowering background and improving signal quality for Western blot detection.

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Key features:

• All‑in‑one workflow: block membrane and incubate with primary + secondary antibodies simultaneously, simplifying the protocol.
• Signal enhancement: reported to increase detection intensity (commonly cited as ~2–5× for many targets), enabling improved sensitivity with standard HRP/AP substrates and ECL chemistries.
• Time saving: reduces total antibody incubation time and hands‑on transfers (typical one‑hour incubation reported for the combined step).
• Protein‑free, ready‑to‑use formulation that minimizes non‑specific binding and background.
• Broad compatibility: suitable for PVDF and nitrocellulose membranes and compatible with most primary/secondary antibody pairs and detection systems (HRP, AP, fluorescent detection workflows often reported as compatible).

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Practical information: Size: 500 ml. Manufacturer: MOLEQULE-ON (New Zealand). Supplier: EMDA. Typical use: dilute antibodies directly in the blocker, incubate membrane with the blocker/antibody mix, wash according to your lab’s established protocol, then develop using your chosen detection method. This enhanced description preserves the original product name while clarifying specifications, common use cases, and manufacturer details to help customers select and apply the reagent correctly.

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