Lysis Buffer (Protein)

Lysis Buffer (Protein) — Enhanced description: Lysis Buffer (Protein) from MOLEQULE-ON is a ready-to-use protein extraction buffer formulated to improve the original description by specifying typical applications and handling. It is designed for efficient solubilization of cellular proteins from cultured cells and tissue for downstream analyses including SDS-PAGE/Western blot, immunoprecipitation, ELISA and protein quantitation assays.

• Manufacturer: MOLEQULE-ON, New Zealand.
• Supplier: EMDA.
• Size: 100 ml.
• Typical applications: total cell lysates, membrane and nuclear protein extraction, sample preparation for Western blot, immunoprecipitation, ELISA and colorimetric/fluorometric protein assays.

Usage notes and compatibility: Formulations for protein lysis buffers commonly include buffering salts and detergents to disrupt membranes and solubilize a broad range of proteins; performance may vary with detergent strength (e.g., NP-40 vs. RIPA-type detergents) and target protein location, so optimization may be required. Add fresh protease and phosphatase inhibitors immediately prior to use, perform lysis on ice (4°C) to limit proteolysis and clarify lysates by centrifugation before downstream assays.

Handling and safety: Store refrigerated per manufacturer guidance (typical working practice: 2–8°C), avoid repeated freeze–thaw cycles, use appropriate PPE and dispose of waste according to local regulations. For best results, follow volume and incubation guidelines appropriate to cell type and downstream application and consult technical notes or the manufacturer for application-specific recommendations.