Human Neutrophin 4

Recombinant Human Neutrophin 4 — enhanced recombinant protein formulation of Neurotrophin-4 (NT-4/5) for neurobiology research, retaining the original identity while clarifying common names and uses. This product is manufactured by MOLEQULE-ON (New Zealand) and supplied by EMDA.

Summary: Recombinant Human Neutrophin 4 is a lyophilized, E. coli-expressed neurotrophic factor (NT-4) that supports neuronal survival, differentiation, and TrkB pathway studies; it is commonly used as a cell culture supplement, positive control in ELISA/Western assays, and in bioactivity/differentiation assays. (prospecbio.com)

• Size / Form: 10 µg, lyophilized powder.
• Source & Purity: E. coli-expressed, typically >95% purity by SDS-PAGE/HPLC; monomer ~14 kDa, dimer ~28-29 kDa. (sigmaaldrich.com)
• Reconstitution & Working Conc: Reconstitute in sterile water (typical stock ~0.1 mg/mL); working concentrations for cellular assays commonly range 20-100 ng/mL depending on assay and cell type. Addition of 0.1% BSA/HSA is recommended for long-term storage of aliquots. (cellsignal.com)
• Storage & Handling: Store lyophilized material at ≤-20°C; after reconstitution aliquot and store at -20°C to -80°C, avoiding repeated freeze-thaw cycles. Handle as a research reagent; follow standard aseptic technique. (medchemexpress.eu)
• Applications: neuronal survival/proliferation assays, differentiation protocols, TrkB signaling studies, assay controls (WB/ELISA), and other in vitro research applications.

Notes: Maintains and expands the original description while clarifying recommended handling, typical concentrations, and intended research uses. For experimental protocols and exact storage/reconstitution instructions follow the manufacturer’s datasheet and batch Certificate of Analysis. For research use only — not for human or diagnostic use.