dNTPs Mix, 25mM

dNTPs Mix, 25mM — premixed solution of dATP, dCTP, dGTP, and dTTP, each at 25 mM; supplied at 1 mL volume. Manufacturer: MOLEQULE-ON (New Zealand). (molequle-on.com)

• Overview & composition: High-quality PCR-grade dNTP mix in a Tris-buffered solution (manufacturer formulation), provided ready-to-dilute for PCR, sequencing, cDNA synthesis, fill-in, and tailing reactions. (molequle-on.com)
• Key features: nuclease-free, high purity suitable for routine and high-fidelity PCR workflows; minimal contamination risk and long-term stability when stored correctly. (thermofisher.com)
• Storage & handling: store frozen at -20°C; avoid repeated freeze–thaw cycles and thaw gently before use. (thermofisher.com)
• Typical applications: endpoint PCR, qPCR/RT-PCR, long-range PCR, sequencing, cDNA synthesis, LAMP/MDA, and other enzymatic incorporation assays. (thermofisher.com)
• Usage guidance (practical): typical final concentration in PCR is 200 μM of each dNTP (0.2 mM each). For example, to reach 200 μM each in a 50 μL reaction from a 25 mM stock, add ~0.4 μL of the 25 mM mix. For small reaction volumes or to improve pipetting accuracy, prepare a diluted working solution (e.g., 2.5–10 mM) and aliquot larger volumes. (neb.com)

Practical tip: because pipetting sub-microliter volumes can reduce accuracy, make a convenient intermediate dilution (for example, 10x) from the 25 mM stock, aliquot single-use portions, and keep frozen to minimize freeze–thaw cycles.

Compatible with standard DNA polymerases and master mixes; intended for research use only.