Commassie Brilliant Blue R-250, Cas # 6104-59-2

Commassie Brilliant Blue R-250, Cas # 6104-59-2 — enhanced description below. (Note: commonly spelled Coomassie Brilliant Blue R-250)

Manufacturer: MOLECULAR, New Zealand

Pack size: 25 g

Overview: A triphenylmethane protein dye primarily used for visualization of protein bands after electrophoresis. It forms intensely colored complexes with proteins (colour change to intense blue on binding) and is widely used for routine SDS-PAGE gel staining due to good sensitivity and reversible binding characteristics. (sigmaaldrich.com)

Key features & specifications:

• CAS: 6104-59-2; molecular weight ~826 g·mol⁻¹.
• Appearance: dark purplish/blue powder; soluble to give dark blue/black solutions in aqueous and acidic organic media.
• Common formulation: typically supplied as a dry dye to be prepared as an acidic staining solution (commonly 25–50% organic solvent such as methanol or ethanol with acetic acid) for gel staining; destaining with methanol/acetic acid or water removes unbound dye.
• Performance: suitable for detection of protein bands in gels (reported detection limits in routine staining down to ~0.5 μg/cm² under typical protocols).

Usage notes & handling: For laboratory research use—follow the supplied Safety Data Sheet and laboratory safety practices. Store dry, protected from strong light and moisture at ambient temperature. Prepare staining and destaining solutions freshly and dispose of solvent-based waste according to local regulations. (cephamls.com)

Compatibility & applications: Optimized for SDS-PAGE gel staining and general protein visualization in electrophoretic and microscopy workflows; not intended for diagnostic or therapeutic use. (sigmaaldrich.com)